pepscanPepScan
specimen

#0064

status: failed
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sequence
AMALDLPGIPCVKKAKQTHVVILQTDDEKGVAAHATTLGNRTCLDRAKGGLDNRRLT
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence48%
confidence 52% · band 36-60%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk31%
confidence 56% · band 20-42%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden37%
confidence 84% · band 33-41%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk5%
confidence 84% · band 1-9%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk27%
confidence 56% · band 16-38%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_37580185a2ae40689d2d3fc8edfe014c
seq sha256: 0d866545b27131cf5d040258ae368d37593cafc88b088ca9f8a99c19b0b3b138
report sha256: ed527981da223256020f9fc6180aea750bcba041727bcfd33a737010317778a2
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“57 residues of pure loop. no helix, no sheet, just a long floppy ribbon doing whatever it wants. mixed composition, nothing dominant, nothing committing. structurally it's a shrug.”
device photo
device photo for specimen #64
created
Tue, 16 Jun 2026 04:25:21 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, multiple cysteines; disulfide heterogeneity risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 2 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 48% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.