pepscanPepScan
specimen

#0050

status: complete
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sequence
ELLQQQEPLLSERAAVVMVLHLAPEFYDIKSMQLNIVVNLKNVCNIISTQFLLLHGYPMTKAD
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence48%
confidence 52% · band 36-60%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk39%
confidence 56% · band 28-50%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden51%
confidence 84% · band 47-55%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk3%
confidence 84% · band 0-7%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk35%
confidence 56% · band 24-46%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_652cfb1e3ad04be1bce2e9008f29d5f8
seq sha256: 8263d4ff0419841dd6f358611e147b112237670a832e5d7f6005d95e70fab3a0
report sha256: a9e91d8811fef6c307572ae382222b74798f7496376d0792385ffbc0089629cb
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“63 residues of pure loop. no structure at all, just a long floppy string drifting through space. weirdly hydrophobic for something so unstructured, like it wants to fold but never got the memo.”
device photo
device photo for specimen #50
created
Tue, 16 Jun 2026 04:11:50 GMT
completed
Tue, 16 Jun 2026 04:36:52 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, long hydrophobic run may increase aggregation risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 2 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 48% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.