pepscanPepScan
specimen

#0459

status: complete
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sequence
EFVMRCVPFKENLAVPGDDRDKYHIVNLTSMTNANIKLARIEQSDITYAKSRTSIFLSIVVL
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence39%
confidence 52% · band 27-51%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk36%
confidence 56% · band 25-47%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden45%
confidence 84% · band 41-49%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk2%
confidence 84% · band 0-6%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk31%
confidence 56% · band 20-42%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_f702fabcd4d94334a7e9f48cd644cca4
seq sha256: 54b1e0d0816ae72216027f619758ff98a92fbb168ca095519afedc5b2999d3cc
report sha256: 08a1eec12764cc54ca8233aa60e24eb8365bfcba3bbcd34fe6264093787b18c6
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“62 residues of pure loop. no structure, no commitment, just a long floppy ribbon doing whatever it wants. unusual for something this size to give up on folding entirely.”
device photo
device photo for specimen #459
created
Sat, 20 Jun 2026 13:59:17 GMT
completed
Sat, 20 Jun 2026 14:08:23 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 1 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 39% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.