pepscanPepScan
specimen

#0458

status: complete
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sequence
GCKLLYTWSVRDLTGSDPISGPTRRESKKVVPSVINSNIKYNVSHNAKRQRTDMDLDPKVEI
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence49%
confidence 52% · band 37-61%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk29%
confidence 56% · band 18-40%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden31%
confidence 84% · band 27-35%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk7%
confidence 84% · band 3-11%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk25%
confidence 56% · band 14-36%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_46a91ceebdd447b8b6f66498f867cd3a
seq sha256: de84cc876ac931d4e1111e69cae00d625727897bf2ca6fcf025e23c4859d03fd
report sha256: 73f0e8f2e89ea7939ba90ac6b8a7f04a288e6f8d89d0bae710f820f0cbf9ee90
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“62 residues of pure loop. no helix, no sheet, just a long floppy string drifting through space. lots of charged residues scattered around, which tracks. this thing has no opinions about its own shape.”
device photo
device photo for specimen #458
created
Sat, 20 Jun 2026 13:58:43 GMT
completed
Sat, 20 Jun 2026 14:05:58 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 1 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 49% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.