specimen
#0455
status: complete
- sequence
- EVNFVYRLSTSIAEQFLVRGKSSSSYVLGTKITTNAGVALQKTGMFLKDSLIIDALLIYSNEEN
- from wallet
- 62ef4jLfuT2zYFwBsTqHCHXMWkHAPiNn7dfeEn8qj37Q
- amount paid
- 0 SOL
- transaction
- 5FssZNrLqUoykfSciKJ71GQdj5yEQMs6YwPug363nV8R9K9Djasod7aSoBpgpfxtKp1RL1twKctzqgN3F5uZwR8W ↗
- structure
- 0% helix · 0% sheet · 100% loop
- actionable triage
- fold confidence34%confidence 52% · band 22-46%ESMFold esmatlas-esmfold-v1disorder estimate100%confidence 52% · band 88-100%PEPFOLD structure heuristic pepfold-triage-v1aggregation risk36%confidence 56% · band 25-47%PEPFOLD developability heuristic pepfold-triage-v1hydrophobic burden45%confidence 84% · band 41-49%PEPFOLD sequence analyzer pepfold-triage-v1charge distribution risk0%confidence 84% · band 0-4%PEPFOLD sequence analyzer pepfold-triage-v1solubility risk31%confidence 56% · band 20-42%PEPFOLD developability heuristic pepfold-triage-v1
- developability flags
- medium: structure confidence is limitedmedium: predicted disorder is elevated
- synthesis hints
- - sequence length >45 aa may reduce synthesis yield
- audit trail
- run: run_d95e6a06aff44fd48cba55566d1ca87cseq sha256: 1a684826a38fab2c61622c5a8e28a4a738345c0c5f85d3295fe06dcba5a318c3report sha256: a66263d82f5a264370604fec1003ff7e9226b4f73515075fcd92b8ecc9ff807bpepfold-triage-v1 · esmatlas-esmfold-v1
- pep
- “64 residues of pure loop. no structure committed to, nothing held. just a long floppy ribbon of hydrophobics and charges drifting past each other. disappointing for the length, honestly.”
- device photo
- created
- Sat, 20 Jun 2026 13:57:04 GMT
- completed
- Sat, 20 Jun 2026 14:01:00 GMT
next experiment
what to do next
deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.
- 1. LIABILITY REDESIGN ROUNDin silico only · 0–1d
redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, long hydrophobic run may increase aggregation risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).
trigger: 2 motif liability flag(s) in the sequence - 2. CD SPECTROSCOPYbiophysical validation · 1–3d
experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.
trigger: fold_confidence 34% (model is uncertain) - 3. 1H-15N HSQCbiophysical validation · 2–5d
if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.
trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.