pepscanPepScan
specimen

#0042

status: complete
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sequence
YGGFL
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence60%
confidence 52% · band 48-72%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk56%
confidence 56% · band 45-67%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden60%
confidence 84% · band 56-64%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk0%
confidence 84% · band 0-4%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk45%
confidence 56% · band 34-56%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: predicted disorder is elevated
synthesis hints
  • - high gly/pro content may affect folding kinetics
audit trail
run: run_4b1b6b4acec648ecb52b28e7d74840bd
seq sha256: 13201d02b42ae510b65ffbd04207e527df07731724d67b90eea59e2f95edcba4
report sha256: 69d37487702f76beb25765b68b1a0e101cec04685b3507e1d17c556f2ce80dde
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“leu-enkephalin. the classic. five residues of pure loop, no structure to speak of, but it doesn't need any to do what it does at the opioid receptor. small and consequential.”
device photo
device photo for specimen #42
created
Tue, 16 Jun 2026 04:07:22 GMT
completed
Tue, 16 Jun 2026 04:26:22 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. DLS + SEC
    biophysical validation · 1–2d

    measure hydrodynamic radius (DLS) and run size-exclusion to detect oligomers, soluble aggregates, or insoluble species before any binding assay.

    trigger: aggregation_risk 56% (high)
  2. 2. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
  3. 3. SERUM STABILITY + SOLUBILITY CURVE
    biophysical validation · 3–7d

    track peptide concentration in 100% human serum over 0/1/4/24h; in parallel run a solubility titration in PBS. tells you whether the peptide survives long enough to act.

    trigger: hydrophobic_burden 60% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.