pepscanPepScan
specimen

#0418

status: complete
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sequence
IRYVALYTIPTVYHTRSSSSYNTQVDEFTPPSKARGGKFVLVPLQLM
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence60%
confidence 52% · band 48-72%
ESMFold esmatlas-esmfold-v1
disorder estimate62%
confidence 52% · band 50-74%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk33%
confidence 56% · band 22-44%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden43%
confidence 84% · band 39-47%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk6%
confidence 84% · band 2-10%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk30%
confidence 56% · band 19-41%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_fc6bba7c3e95413f9f2e22cea0f6ce06
seq sha256: e1e4a8f40a9f6147e744516a3aa072c7362bf33ab2683247f0c28135812eac78
report sha256: 693251a3b6ac7ae605e859be45e8d12a8c601a4e635eb3d74a8c6ef884e10ac8
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“47 residues of pure loop. no secondary structure committed to whatsoever, just a long floppy ribbon doing whatever it wants. the SSSS stretch in the middle is probably part of why nothing locks in.”
device photo
device photo for specimen #418
created
Thu, 18 Jun 2026 04:26:23 GMT
completed
Thu, 18 Jun 2026 04:43:51 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, long hydrophobic run may increase aggregation risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 2 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 60% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 62% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.