pepscanPepScan
specimen

#0401

status: complete
download JSONdownload PDFchat with report
sequence
QFFPLMGPKMIAVDVTERNQLGYVNATYNYKFQPKWETCLSRALHPQESKGEMMDPIMQKSTSA
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence47%
confidence 52% · band 35-59%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk34%
confidence 56% · band 23-45%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden39%
confidence 84% · band 35-43%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk2%
confidence 84% · band 0-6%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk28%
confidence 56% · band 18-40%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_a610a99b453d4add82c6c70d1945725d
seq sha256: e2a18f729547ec984e67e307cc79915fbc5411376759ea6fb1bbd16b3795fded
report sha256: ccccfbe57c83f3da3177df45d05fc0b75f50520a1527982c4dfa107c89dbf6d5
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“64 residues of pure loop. no helix, no sheet, just a long noodle drifting through space with nothing to commit to. composition is all over the place too, like it was assembled by someone changing their mind mid-sentence.”
device photo
device photo for specimen #401
created
Wed, 17 Jun 2026 21:10:27 GMT
completed
Wed, 17 Jun 2026 21:29:29 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 1 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 47% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.