specimen
#0376
status: complete
- sequence
- GKARCKFMAQLIGNSLARNQTYAPEALLCFRQVAATLLEDEYQMKGDQKFVM
- from wallet
- A2ahw7xcXfzJwDAzkz4vcpHaBR9zseViCr6Ps2GgjFJM
- amount paid
- 0 SOL
- transaction
- 61dGjxvsay5hhBpmz5KBNcpct1NdTUNKxH8967tQFgSqAxzKmQDT1UZvv7cD2n548MoZueGqscY6eRJbjEaRiWrE ↗
- structure
- 0% helix · 0% sheet · 100% loop
- actionable triage
- fold confidence57%confidence 52% · band 45-69%ESMFold esmatlas-esmfold-v1disorder estimate100%confidence 52% · band 88-100%PEPFOLD structure heuristic pepfold-triage-v1aggregation risk37%confidence 56% · band 26-48%PEPFOLD developability heuristic pepfold-triage-v1hydrophobic burden46%confidence 84% · band 42-50%PEPFOLD sequence analyzer pepfold-triage-v1charge distribution risk4%confidence 84% · band 0-8%PEPFOLD sequence analyzer pepfold-triage-v1solubility risk32%confidence 56% · band 21-43%PEPFOLD developability heuristic pepfold-triage-v1
- developability flags
- medium: structure confidence is limitedmedium: predicted disorder is elevated
- synthesis hints
- - sequence length >45 aa may reduce synthesis yield
- audit trail
- run: run_af4e6d1cf168410eb7c467051f89faacseq sha256: 6dcf79171b7ace4a2c0b49e5659cb2679673391f0baa3e78fc2d5aed698dc999report sha256: 6685d2edf2d8e87211f87cc109adc4d6b965daf7b9f13d9bc265a081396a902cpepfold-triage-v1 · esmatlas-esmfold-v1
- pep
- “52 residues of pure loop. no helix, no sheet, just a long floppy ribbon doing nothing in particular. mixed composition, charged and hydrophobic scattered evenly, like it couldn't commit to a personality.”
- device photo
- created
- Wed, 17 Jun 2026 20:03:39 GMT
- completed
- Wed, 17 Jun 2026 20:20:40 GMT
next experiment
what to do next
deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.
- 1. LIABILITY REDESIGN ROUNDin silico only · 0–1d
redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, multiple cysteines; disulfide heterogeneity risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).
trigger: 2 motif liability flag(s) in the sequence - 2. CD SPECTROSCOPYbiophysical validation · 1–3d
experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.
trigger: fold_confidence 57% (model is uncertain) - 3. 1H-15N HSQCbiophysical validation · 2–5d
if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.
trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.