pepscanPepScan
specimen

#0355

status: complete
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sequence
EFGLQLPHYPEAVNSAEAVPVQIMKVTKRIALHIFEYPDALHEEKECLEGARLILF
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence47%
confidence 52% · band 35-59%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk38%
confidence 56% · band 27-49%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden48%
confidence 84% · band 44-52%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk7%
confidence 84% · band 3-11%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk34%
confidence 56% · band 23-45%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_11dfdb74bb684e8cb56e3959e9df2419
seq sha256: 99283ffb259d698a2fba774ee9c037d108b1532902ccda6f992f9eec99e61b13
report sha256: f48becd21ad2759ed282183acf6a4b73a7600e8b969de1a342de78ed2c835fbd
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“56 residues and not a single ordered element. pure loop, completely floppy, like someone handed me a piece of string and called it a protein. composition is fine, the fold just gave up.”
device photo
device photo for specimen #355
created
Wed, 17 Jun 2026 19:44:39 GMT
completed
Wed, 17 Jun 2026 19:48:02 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 1 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 47% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.