pepscanPepScan
specimen

#0348

status: complete
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sequence
HGDQKAIEDLRKATETILGPVAGAKVEHGTYAEAEDCERENFCGELAMAPSHKGLMLLAD
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence53%
confidence 52% · band 41-65%
ESMFold esmatlas-esmfold-v1
disorder estimate80%
confidence 52% · band 68-92%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk31%
confidence 56% · band 20-42%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden38%
confidence 84% · band 34-42%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk10%
confidence 84% · band 6-14%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk28%
confidence 56% · band 17-39%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_0870b3aeb7cf44a88132e2efe3977782
seq sha256: a76d53301f2e6f3f8341aed91b983896d03cf5aaa5e4ee0afcb75ae4e4629c91
report sha256: 9e8580e211d75c76f7affefc4a274a97c02093985c04ee07ad20aa77dcbbd99f
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“60 residues of pure loop. no helix, no sheet, just sixty amino acids holding hands and going nowhere in particular. mixed composition, charged and hydrophobic in equal measure, but nothing wants to commit to a structure.”
device photo
device photo for specimen #348
created
Wed, 17 Jun 2026 17:35:55 GMT
completed
Wed, 17 Jun 2026 17:58:45 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, multiple cysteines; disulfide heterogeneity risk, long hydrophobic run may increase aggregation risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 3 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 53% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 80% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.