pepscanPepScan
specimen

#0324

status: complete
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sequence
NAYSKAVGPLAHAVALSILTCKDYITNYVYAGMYVCIFTFLKFVVRHLLNK
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence68%
confidence 52% · band 56-80%
ESMFold esmatlas-esmfold-v1
disorder estimate63%
confidence 52% · band 51-75%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk41%
confidence 56% · band 30-52%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden59%
confidence 84% · band 55-63%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk8%
confidence 84% · band 4-12%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk38%
confidence 56% · band 27-49%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_508471b2bb074591af9f16f5c61ad7e8
seq sha256: 54c5b14ddca25319b52d31f3c482128282288975b37921bf46c0dc29535ea15c
report sha256: 1b236d5bf9676b6126a9e182d7c10306ffcbec02e8144c77c2c09854abbc6839
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“51 residues and not a single secondary structure element. all loop, completely unstructured, which is suspicious given how hydrophobic the middle gets. should be burying itself somewhere but instead it just flops. weird one.”
device photo
device photo for specimen #324
created
Wed, 17 Jun 2026 17:21:22 GMT
completed
Wed, 17 Jun 2026 17:23:39 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible, multiple cysteines; disulfide heterogeneity risk. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 2 motif liability flag(s) in the sequence
  2. 2. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 63% (high)
  3. 3. SERUM STABILITY + SOLUBILITY CURVE
    biophysical validation · 3–7d

    track peptide concentration in 100% human serum over 0/1/4/24h; in parallel run a solubility titration in PBS. tells you whether the peptide survives long enough to act.

    trigger: hydrophobic_burden 59% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.