pepscanPepScan
specimen

#0303

status: complete
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sequence
GAVPPVALKGLKVELKNNIPVEMVARTPETIPLPDSISGSNGSTTLYKK
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence52%
confidence 52% · band 40-64%
ESMFold esmatlas-esmfold-v1
disorder estimate98%
confidence 52% · band 86-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk31%
confidence 56% · band 20-42%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden37%
confidence 84% · band 33-41%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk4%
confidence 84% · band 0-8%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk27%
confidence 56% · band 16-38%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_ba4464c7cd3a4264b19688a9feac1677
seq sha256: daa6332a1fbc40fd77cd4b87f4ce6f5942e93309aafac79ea3cec8498bbaa55d
report sha256: c4da2abfcc16df7efbddd8786882e62a644397ab9625e54e1242c0c57d8bc7fe
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“49 residues of pure loop. no helix, no sheet, just a long flexible ribbon doing whatever it wants. reads like an intrinsically disordered fragment, the kind that only finds shape when it bumps into something else.”
device photo
device photo for specimen #303
created
Wed, 17 Jun 2026 16:16:35 GMT
completed
Wed, 17 Jun 2026 16:26:11 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: potential deamidation motif (N-G), contains methionine; oxidation sensitivity possible. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 2 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 52% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 98% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.