pepscanPepScan
specimen

#0174

status: complete
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sequence
NKAQASPRRFDSPAIHIIDPMAKPIHTTCAAAVGTGYDLPVESALSTQVIPISAHLLGSFFLI
amount paid
0 SOL
structure
0% helix · 0% sheet · 100% loop
actionable triage
fold confidence41%
confidence 52% · band 29-53%
ESMFold esmatlas-esmfold-v1
disorder estimate100%
confidence 52% · band 88-100%
PEPFOLD structure heuristic pepfold-triage-v1
aggregation risk36%
confidence 56% · band 25-47%
PEPFOLD developability heuristic pepfold-triage-v1
hydrophobic burden46%
confidence 84% · band 42-50%
PEPFOLD sequence analyzer pepfold-triage-v1
charge distribution risk0%
confidence 84% · band 0-4%
PEPFOLD sequence analyzer pepfold-triage-v1
solubility risk31%
confidence 56% · band 20-42%
PEPFOLD developability heuristic pepfold-triage-v1
developability flags
medium: structure confidence is limited
medium: predicted disorder is elevated
synthesis hints
  • - sequence length >45 aa may reduce synthesis yield
audit trail
run: run_aa22fba472ba447fab77ed319ebe1210
seq sha256: ff878cc9572701cf2a43ebc40d3bdcc2808c66c5be282a646035e08f8d193bb9
report sha256: d666142874a2566093d80ed7089b86be720cb4537e9edc553881367c45959d66
pepfold-triage-v1 · esmatlas-esmfold-v1
pep
“63 residues of pure loop. no structure, no commitment, just a long floppy ribbon doing whatever it wants. the hydrophobic tail at the end is interesting though, looks like it wanted to be a membrane anchor and gave up.”
device photo
device photo for specimen #174
created
Tue, 16 Jun 2026 07:23:35 GMT
completed
Tue, 16 Jun 2026 07:50:12 GMT
next experiment

what to do next

deterministic suggestions derived from this specimen's triage report. each entry cites the signal that triggered it. ordered cheapest-first.

  1. 1. LIABILITY REDESIGN ROUND
    in silico only · 0–1d

    redesign to remove the flagged motif(s) before going wet-lab: contains methionine; oxidation sensitivity possible. minimal substitutions usually suffice (e.g. N→Q for deamidation hotspots, M→L for met oxidation).

    trigger: 1 motif liability flag(s) in the sequence
  2. 2. CD SPECTROSCOPY
    biophysical validation · 1–3d

    experimental secondary structure check. confirms whether the predicted helix/sheet content matches a real spectrum before committing to higher-cost assays.

    trigger: fold_confidence 41% (model is uncertain)
  3. 3. 1H-15N HSQC
    biophysical validation · 2–5d

    if disorder is real, peaks will collapse into a narrow proton dispersion. if the peptide is actually folded, peaks will spread out. cheapest way to distinguish IDP from misfold.

    trigger: disorder_estimate 100% (high)
engine pepfold-recs-v1 · not medical advice. use as a starting point for protocol design.