{
  "report_format_version": "pepfold-report-v1",
  "generated_at": "2026-08-05T05:22:03.009Z",
  "specimen": {
    "number": 158,
    "sequence": "RSSANTLFIHLEAIQAEQT",
    "sequence_length": 19,
    "status": "complete",
    "wallet": "DUBfTKUphZhpnW2asrxF93HMZw5wb6mLZSDhZB88kLNV",
    "amount_sol": 0,
    "tx_hash": "5gVKbjzK58i7ajubFpvKmmCr4hbzDKfu6s7qbSPJcLDd54cmWZhgQW3uvAU7qYH9owtdEPws1JSv3uaLEJL9Ua1K",
    "created_at": "2026-06-16T07:06:31.73126+00:00",
    "completed_at": "2026-06-16T07:25:20.474847+00:00"
  },
  "structure": {
    "pdb_url": "https://mrzziawbddodlpywncfy.supabase.co/storage/v1/object/public/pdb-files/67f87b7f-eff9-46e0-bf9c-097b379d299d.pdb",
    "helix_pct": 0,
    "sheet_pct": 0,
    "loop_pct": 100
  },
  "triage": {
    "models": {
      "fold_model": {
        "name": "ESMFold",
        "version": "esmatlas-esmfold-v1"
      },
      "triage_model": {
        "name": "PEPFOLD heuristic triage",
        "version": "pepfold-triage-v1"
      },
      "comment_model": {
        "name": "Claude",
        "version": "claude-opus-4-7"
      }
    },
    "run_id": "run_d07003ebdee344eaae58caf9cf9c16bb",
    "metrics": [
      {
        "id": "fold_confidence",
        "label": "fold confidence",
        "value": 0.851,
        "source": {
          "model": "ESMFold",
          "version": "esmatlas-esmfold-v1"
        },
        "confidence": 0.52,
        "evidence_note": "derived from mean pLDDT on CA atoms",
        "confidence_band": [
          0.731,
          0.971
        ],
        "not_enough_confidence": true
      },
      {
        "id": "disorder_estimate",
        "label": "disorder estimate",
        "value": 0.053,
        "source": {
          "model": "PEPFOLD structure heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.52,
        "evidence_note": "estimated from loop proportion and low-confidence structure regions",
        "confidence_band": [
          0,
          0.173
        ],
        "not_enough_confidence": true
      },
      {
        "id": "aggregation_risk",
        "label": "aggregation risk",
        "value": 0.247,
        "source": {
          "model": "PEPFOLD developability heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.56,
        "evidence_note": "hydrophobic burden, aromatic burden, charge, and long hydrophobic runs",
        "confidence_band": [
          0.137,
          0.357
        ],
        "not_enough_confidence": false
      },
      {
        "id": "hydrophobic_burden",
        "label": "hydrophobic burden",
        "value": 0.421,
        "source": {
          "model": "PEPFOLD sequence analyzer",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.84,
        "evidence_note": "fraction of hydrophobic residues in sequence",
        "confidence_band": [
          0.381,
          0.461
        ],
        "not_enough_confidence": false
      },
      {
        "id": "charge_distribution_risk",
        "label": "charge distribution risk",
        "value": 0.053,
        "source": {
          "model": "PEPFOLD sequence analyzer",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.84,
        "evidence_note": "absolute net charge normalized by sequence length",
        "confidence_band": [
          0.013,
          0.093
        ],
        "not_enough_confidence": false
      },
      {
        "id": "solubility_risk",
        "label": "solubility risk",
        "value": 0.247,
        "source": {
          "model": "PEPFOLD developability heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.56,
        "evidence_note": "composite of aggregation risk, hydrophobic burden, and charge",
        "confidence_band": [
          0.137,
          0.357
        ],
        "not_enough_confidence": false
      },
      {
        "id": "developability_risk",
        "label": "developability risk",
        "value": 0.208,
        "source": {
          "model": "PEPFOLD developability heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.56,
        "evidence_note": "composite developability indicator used for triage",
        "confidence_band": [
          0.098,
          0.318
        ],
        "not_enough_confidence": false
      }
    ],
    "generated_at": "2026-06-16T07:24:02.104715+00:00",
    "report_version": "phase-a.v1",
    "sequence_length": 19,
    "synthesis_hints": [],
    "motif_liabilities": [],
    "developability_flags": [],
    "low_confidence_reasons": [
      "DSSP unavailable; secondary structure uses pLDDT fallback."
    ]
  },
  "audit": {
    "input": {
      "sequence_length": 19,
      "sequence_sha256": "b0b606059be3647a2bbfdeb75225a03a3009cc31d255bb49937fb803074cc511"
    },
    "output": {
      "report_sha256": "d6d49e100469dc81d32cb9d0643a1daf61acb490cb94495660740730e3d54475"
    },
    "run_id": "run_d07003ebdee344eaae58caf9cf9c16bb",
    "timestamp": "2026-06-16T07:24:02.104827+00:00",
    "pipeline_versions": {
      "pep_model_version": "claude-opus-4-7",
      "fold_model_version": "esmatlas-esmfold-v1",
      "triage_model_version": "pepfold-triage-v1",
      "secondary_structure_source": "plddt_fallback"
    }
  },
  "attestation": null,
  "next_experiments": {
    "engine": "pepfold-recs-v1",
    "recommendations": [
      {
        "id": "functional_assay",
        "technique": "TARGET-SPECIFIC BINDING ASSAY",
        "rationale": "no structural or developability red flags. profile is good enough to spend reagent on a real assay against your intended target (SPR, BLI, MIC, cytotox, etc., depending on context).",
        "evidence": "no developability flags · fold confidence ≥ 75% · low aggregation / disorder",
        "turnaround_days": [
          5,
          14
        ],
        "tier": "binding_or_function"
      }
    ]
  },
  "comment": "19 residues, zero secondary structure, all loop. nothing wants to fold here, just a floppy stretch of mixed polar and hydrophobic residues drifting through space. reads like a disordered linker that got submitted by mistake.",
  "tweet": {
    "id": "2066784171994005850",
    "url": "https://x.com/pepfoldagent/status/2066784171994005850"
  }
}