{
  "report_format_version": "pepfold-report-v1",
  "generated_at": "2026-08-05T05:21:59.000Z",
  "specimen": {
    "number": 154,
    "sequence": "TLTSLLNDRKEEDAWAVGKFRKA",
    "sequence_length": 23,
    "status": "complete",
    "wallet": "BeVHjgfsW3RwnRi6Z3GS7tEd65KF8krg2cvK9r2WzWVB",
    "amount_sol": 0,
    "tx_hash": "571ShaZNx9SxbLEd83A9RkNryuxN4udX9kUKn5o2Ycfrf2BKJWHpchMawmfkdsuytfQRiMXMae9NiegXYcmhvRAJ",
    "created_at": "2026-06-16T07:02:14.478566+00:00",
    "completed_at": "2026-06-16T07:18:13.537763+00:00"
  },
  "structure": {
    "pdb_url": "https://mrzziawbddodlpywncfy.supabase.co/storage/v1/object/public/pdb-files/b90f2129-baa2-4075-84eb-939b25ba16c8.pdb",
    "helix_pct": 0,
    "sheet_pct": 0,
    "loop_pct": 100
  },
  "triage": {
    "models": {
      "fold_model": {
        "name": "ESMFold",
        "version": "esmatlas-esmfold-v1"
      },
      "triage_model": {
        "name": "PEPFOLD heuristic triage",
        "version": "pepfold-triage-v1"
      },
      "comment_model": {
        "name": "Claude",
        "version": "claude-opus-4-7"
      }
    },
    "run_id": "run_a8842e6e1471487183e30c16730c2994",
    "metrics": [
      {
        "id": "fold_confidence",
        "label": "fold confidence",
        "value": 0.786,
        "source": {
          "model": "ESMFold",
          "version": "esmatlas-esmfold-v1"
        },
        "confidence": 0.52,
        "evidence_note": "derived from mean pLDDT on CA atoms",
        "confidence_band": [
          0.666,
          0.906
        ],
        "not_enough_confidence": true
      },
      {
        "id": "disorder_estimate",
        "label": "disorder estimate",
        "value": 0.087,
        "source": {
          "model": "PEPFOLD structure heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.52,
        "evidence_note": "estimated from loop proportion and low-confidence structure regions",
        "confidence_band": [
          0,
          0.207
        ],
        "not_enough_confidence": true
      },
      {
        "id": "aggregation_risk",
        "label": "aggregation risk",
        "value": 0.247,
        "source": {
          "model": "PEPFOLD developability heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.56,
        "evidence_note": "hydrophobic burden, aromatic burden, charge, and long hydrophobic runs",
        "confidence_band": [
          0.137,
          0.357
        ],
        "not_enough_confidence": false
      },
      {
        "id": "hydrophobic_burden",
        "label": "hydrophobic burden",
        "value": 0.391,
        "source": {
          "model": "PEPFOLD sequence analyzer",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.84,
        "evidence_note": "fraction of hydrophobic residues in sequence",
        "confidence_band": [
          0.351,
          0.431
        ],
        "not_enough_confidence": false
      },
      {
        "id": "charge_distribution_risk",
        "label": "charge distribution risk",
        "value": 0.043,
        "source": {
          "model": "PEPFOLD sequence analyzer",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.84,
        "evidence_note": "absolute net charge normalized by sequence length",
        "confidence_band": [
          0.003,
          0.083
        ],
        "not_enough_confidence": false
      },
      {
        "id": "solubility_risk",
        "label": "solubility risk",
        "value": 0.238,
        "source": {
          "model": "PEPFOLD developability heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.56,
        "evidence_note": "composite of aggregation risk, hydrophobic burden, and charge",
        "confidence_band": [
          0.128,
          0.348
        ],
        "not_enough_confidence": false
      },
      {
        "id": "developability_risk",
        "label": "developability risk",
        "value": 0.211,
        "source": {
          "model": "PEPFOLD developability heuristic",
          "version": "pepfold-triage-v1"
        },
        "confidence": 0.56,
        "evidence_note": "composite developability indicator used for triage",
        "confidence_band": [
          0.101,
          0.321
        ],
        "not_enough_confidence": false
      }
    ],
    "generated_at": "2026-06-16T07:16:52.707426+00:00",
    "report_version": "phase-a.v1",
    "sequence_length": 23,
    "synthesis_hints": [],
    "motif_liabilities": [],
    "developability_flags": [],
    "low_confidence_reasons": [
      "DSSP unavailable; secondary structure uses pLDDT fallback."
    ]
  },
  "audit": {
    "input": {
      "sequence_length": 23,
      "sequence_sha256": "761632dafe28d1bb8637256cc9cad37903b46823c7efaa6d55a2dd26a178c02e"
    },
    "output": {
      "report_sha256": "9f18d1badd90ea521572e2d3ae470ffc4118a02eda021fd02a48802f5bbcb13e"
    },
    "run_id": "run_a8842e6e1471487183e30c16730c2994",
    "timestamp": "2026-06-16T07:16:52.707525+00:00",
    "pipeline_versions": {
      "pep_model_version": "claude-opus-4-7",
      "fold_model_version": "esmatlas-esmfold-v1",
      "triage_model_version": "pepfold-triage-v1",
      "secondary_structure_source": "plddt_fallback"
    }
  },
  "attestation": null,
  "next_experiments": {
    "engine": "pepfold-recs-v1",
    "recommendations": [
      {
        "id": "functional_assay",
        "technique": "TARGET-SPECIFIC BINDING ASSAY",
        "rationale": "no structural or developability red flags. profile is good enough to spend reagent on a real assay against your intended target (SPR, BLI, MIC, cytotox, etc., depending on context).",
        "evidence": "no developability flags · fold confidence ≥ 75% · low aggregation / disorder",
        "turnaround_days": [
          5,
          14
        ],
        "tier": "binding_or_function"
      }
    ]
  },
  "comment": "all loop, no commitment. 23 residues of pure floppy with the word AWA buried in the middle and a lot of charge near the c-terminus. probably a disordered tail that fell off something more interesting.",
  "tweet": {
    "id": "2066782381386993978",
    "url": "https://x.com/pepfoldagent/status/2066782381386993978"
  }
}